Fig. 2
Trodusquemine promotes the formation of shorter and thicker Aβ42 fibrils in vitro. AFM (a, b) and TEM (c, d) measurements of Aβ42 in the absence (left panels) or presence (right panels) of an equimolar concentration of trodusquemine. Samples were prepared at the initiation stage (t = 0 h, a, c) and in the plateau region (t = 4 h, b, d) of the aggregation reaction. Scale bars, 500 nm. AFM distributions of fibril lengths (e) (N = 100 per condition, ***P < 0.001 by paired t-test) and cross-sectional heights (f) (N = 387 for Aβ42 and N = 174 for Aβ42 + 1:1 TRO, ***P < 0.001 by paired t-test), and TEM widths (g) (N = 200 per condition, ***P < 0.001 by paired t-test) in the absence (black) and presence (blue) of trodusquemine. In e–g the square denotes the mean, the centre line indicates the median, the bounds of the boxes indicate the first and third quartiles containing 50% of the data, and the whiskers indicate the first standard deviation
