Fig. 10
From: Regulation of mycobacterial infection by macrophage Gch1 and tetrahydrobiopterin
Gch1 expression inversely correlates with control of BCG infection in vitro. a, b A mycobacterial growth inhibition assay was performed using Nos2−/− (a) and Gch1−/ (b) BMDM cells. Macrophages were infected with 50:1 MOI BCG in the presence of 10 ng/ml IFNγ and the live BCG remaining after 96 h was enumerated by the BACTEC Mycobacterial Growth Indicator Tube (MGIT) system (MGIA), n = 6 per genotype Nos2+/+ and Nos2−/−, n = 5 Gch1fl/fl and n = 5 Gch1fl/flTie2cre n = 7 p < 0.05 (t-test), individual points with SEM shown. Δlog10CFU = log10 CFU sample − log10 CFU positive control. Error bars are ±SEM. c, d Pearson correlation between GCH1 expression and in vitro BCG growth in PBMCs of 78 BCG vaccinated South African infants. Points represent the mean of duplicate cultures. Δlog10CFU (M.BCG) = log10 CFU of sample − log10 CFU of control
