Fig. 4

Absence of Gch1 alters macrophage gene expression in response to infection. Macrophages were infected with BCG (MOI 1:1)/IFNγ (10 ng/ml) for 24 h or incubated for 24 h in the absence of infection (n = 4 per genotype). RNA was extracted and a whole mouse genome gene expression array was performed. a Genes were determined as significantly regulated by genotype by comparison to their relevant wildtype control with p < 0.05 (adjusted for multiple testing). The number of genes significantly regulated by genotype for each condition was calculated. The number of genes shared between genotypes and conditions is shown by Venn Diagram. b Selected relevant gene functional annotations determined with significantly increased or decreased expression in BCG infected wildtype vs Gch1^fl/flTie2cre macrophages, as determined using IPA analysis with Z score > 2 and a p-value for overlap p < 0.01. c Heatmaps for genes within individual gene functional annotations determined in (c, d). d Selected relevant predicted upstream regulators with significantly increased or decreased activation in BCG infected wildtype vs Gch1^fl/flTie2cre macrophages, as determined using IPA analysis with Z score > 2 and a p-value for overlap p < 0.01