Fig. 5 | Nature Communications

Fig. 5

From: Regulation of mycobacterial infection by macrophage Gch1 and tetrahydrobiopterin

Fig. 5

Divergent gene expression changes in BH4 and iNOS-deficient macrophages. BMDM were prepared from Gch1fl/fl,  Gch1fl/flTie2cre, Nos2+/+ and Nos2−/− age-matched animals. Macrophages were infected at a MOI of 1:1 with BCG in the presence of 10 ng/ml IFNγ, or left uninfected followed by RNA extraction. Gene expression was quantified using TaqMan gene expression assays. GOI Gene of Interest. Data was analysed by two-tailed T-test * p < 0.05, error bars ± SEM

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