Fig. 1 | Nature Communications

Fig. 1

From: Proteasomal degradation of NOD2 by NLRP12 in monocytes promotes bacterial tolerance and colonization by enteropathogens

Fig. 1

NLRP12 interacts with NOD2 through a linker-region proximal to the nucleotide-binding domain. a Co-immunoprecipitation with anti-Myc (top panel) and lysates (bottom panel) of the THP-1 Myc-BirA*-NOD2 stable cell line that were treated with muramyl dipeptide (MDP at 10 µg/mL) or lipopolysaccharide (LPS at 0.2 µg/mL) for two h. b Scheme of Myc-tagged constructs encoding human wild-type NLRP12 and mutated NLRP12 proteins Δ-PYD linker (200 aa–1061 aa), 1–224 aa and 1–98 aa. c Western blot analysis of NOD2–RIPK2 complexes that were precipitated in the presence of increasing amounts of NLRP12 in HEK293T cells. d Representative co-immunoprecipitation experiment with indicated plasmids. Immunoprecipitation was performed on native protein lysates by using antibodies against FLAG (M2). NOD2-specific immunoprecipitation was confirmed by western blot analysis using anti-Flag antibody. NLRP12 and NOD2 were detected using anti-Myc 9E10 and anti-FLAG (M2) antibody respectively. Non-precipitated protein lysates were used as input controls

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