Fig. 6: LYVE1 is expressed by both yolk sac and embryo cells at E8.5.

a Representative flow cytometry plots of LYVE1 expression in freshly isolated E8.5 (5–7sp) embryos and YS. b Cell surface expression of LYVE1 in YS and embryos dissected from freshly isolated E8.5 (5–7sp) concepti (n = 16). The absolute number per concepti and frequency as a percent of total live events of LYVE1^low and LYVE^high cells is shown. Each circle represents an independent YS or embryo. c Frequency of CD41⁺ cells as a percent of total live events of LYVE1^low and LYVE^high cells is shown (n = 16) for embryos and YS. a–c Two independent litters were analyzed. (***p < 0.001, two-sample t-test for LYVE1^low and eWrs-test LYVE^high; differences held even after multiple testing corrections to control a FDR of 0.05). Means are shown. Error bars denote standard deviation. d Representative confocal images showing the pattern of expression of LYVE1 in E8.5 embryos and YS. Two independent embryos are shown. For each representative embryo an inset zoomed on one of the paired aortas is also shown. Anti-LYVE1 conjugated to CF^TM568 is shown in red and anti-CD31⁻BV421 is shown in blue. Scale bars stand for 50 µm for the low magnification (i and iii) and 10 µm for the high magnification (ii and iv). Schematics indicate anatomical parts: FG: foregut; A: paired-aorta; NT: Neural tube. Source data are provided as a Source Data file