Fig. 3

Has1, Mak5 and Spb4 crosslink to spatially distinct pre-rRNA sequences. a Wild-type yeast (WT) or yeast expressing C-terminally HTP tagged Has1, Spb4 or Mak5 were subjected to PAR-CRAC and the obtained sequence reads were mapped to the S. cerevisiae genome. The relative proportions of reads mapping to different classes of RNAs is shown. b For WT, Has1-HTP, Mak5-HTP and Spb4-HTP, the normalised number of reads mapped per nucleotide to the RDN37 locus is shown above a schematic view of the initial 35S pre-rRNA transcript. Nucleotide positions on the 35S pre-rRNA transcript are indicated below the graph. c–f The number of T-C mutations detected per nucleotide of the RDN37 locus is shown for the WT (c), Has1-HTP (d), Mak5-HTP (e) and Spb4-HTP (f) CRAC samples. ETS external transcribed spacer, ITS internal transcribed spacer