Fig. 2
From: Enhanced astrocyte responses are driven by a genetic risk allele associated with multiple sclerosis
rs7665090G variant effect on human iPSC-derived astrocytes. a, b Degradation of IκBα and phosphorylation of p65 in iPSC-derived astrocytes with the risk or protective variants at a resting state and 10 min after stimulation with TNFα (50 ng/ml) and IL-1β (10 ng/ml) by flow cytometry. c Expression of p50 and p65 in unstimulated and stimulated (50 ng/ml TNFα and 10 ng/ml IL-1β for 48 h) iPSC-derived astrocytes from both groups by western blot. d Volcano plot profiling astroglial expression of 84 NF-κB target genes after stimulation with TNFα, IL-1β, and IFNγ for 16 h. Red dots indicate genes with ≥2-fold expression and FDR-adjusted p-values ≤ 0.05. e Protein expression corresponding to genes with significantly increased expression. f Uptake of L-[3,4-3H] glutamic acid by iPSC-derived unstimulated and stimulated (with TNFα and IL-1β) astrocytes with the risk or protective genotype. g, h Glucose uptake and lactate secretion by astrocytes with the risk or protective variants. Data represents means ± s.d. from three independent experiments. p-Values shown for the post-hoc Tukey–Kramer test performed after one-way ANOVA. *p < 0.05, **p < 0.01, and ***p < 0.001
