Fig. 1
From: Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development
Germline-expressed miRNAs are largely drosha-independent. a FirePlex® assay-based heat map analysis of miRNAs on dissected wild-type and drosha(ok369) germlines at 20 °C. Values/colors reflect the mean fluorescent intensity (MFI). Deep blue = low miRNA accumulation. Bright orange = high miRNA accumulation. Red boxes: miRNAs that are reduced in drosha(ok369) germlines termed drosha-dependent. Blue boxes: miRNAs that accumulate in drosha(ok369) were termed drosha-independent. One-hundred dissected gonads were assayed for each genotype in three biological replicates. b, c TaqMan™ analysis on wild-type, drosha(ok369) and each of the miRNA mutant genotypes at 18 h past L4. drosha-dependent miRNAs do not accumulate in drsh-1 mutants (b), while drosha-independent miRNAs are still expressed (c). Each TaqMan™ assay was performed three times on 100 whole worms each time for each genotype. Statistical significance was calculated by one-way ANOVA with the Bonferroni’s correction. ****P < 0.0001. ± SD
