Fig. 4

RNAi mediated depletion of oocyte-expressed miRNAs reveals defects in meiotic progression and oocyte development. The photograph displays live imaging of germlines from whole animals bearing membrane GFP (green) and Histone 2B mCherry (red). The animal is oriented in dorsal (top) ventral (bottom) polarity. The schematic in each panel depicts the pattern of accumulation, gray indicates regions of positive localization. a Luciferase RNAi does not produce oocyte defects. See also Supplementary Fig. 12 for negative controls. b mir-35–41 RNAi results in a lower number of oocytes and delayed pachytene progression. c mir-61 RNAi results in a lower number of oocytes and delayed pachytene progression. d mir-51 RNAi causes oocyte disorganization and double row of diplotene oocytes, coupled with lower oocyte number. e mir-72 RNAi results in a lower number of oocytes. f mir-54–56 RNAi results in a lower number of oocytes. g Quantitation of oocyte number from each of the RNAi treatments. Each oocyte is numbered from diplotene stage (starting at the loop region). Each experiment was performed three times, ~20–25 animals assayed per replicate and statistical significance was calculated by one-way ANOVA with Bonferroni’s correction. ****P < 0.0001, **P < 0.001, *P < 0.01. ±SD. Scale bar: 20 μM. h Percentage of animals displaying pachytene progression defects quantified for each RNAi treatment taking all three replicates into consideration