Table 1 CryoEM data collection, refinement, and validation statistics

From: Structure of the type VI secretion system TssK–TssF–TssG baseplate subcomplex revealed by cryo-electron microscopy

 

TssKSNFG (EMDB-9341) (PDB 6N38)

TssKSN (EMDB-9342)

TssFG (EMDB-9343)

Data collection and processing

Magnification

36,496

36,496

36,496

Voltage (kV)

300

300

300

Electron exposure (e–/Å2)

40

40

40

Defocus range (μm)

0.2–4.0

0.2-4.0

0.2-4.0

Pixel size (Å)

1.37

1.37

1.37

Symmetry imposed

Initial particle images (no.)

369,971

369,971

369,971

Final particle images (no.)

57,567

57,567

57,567

Map resolution (Å)

3.7

3.6

3.9

   FSC threshold

0.143

0.143

0.143

Map resolution range (Å)

Refinement

Model resolution (Å)

3.7

  

   FSC threshold

0.5

  

Model resolution range (Å)

Map sharpening B factor (Å2)

−69

  

Model composition

   Non-hydrogen atoms

24,934

  

   Protein residues

3145

  

   Ligands

R.m.s. deviations

   Bond lengths (Å)

0.013

  

   Bond angles (°)

1.286

  

  Validation

   MolProbity score

0.95

  

   Clashscore

1.02

  

   Poor rotamers (%)

0.07

  

  Ramachandran plot

   Favored (%)

97.20

  

   Allowed (%)

2.67

  

   Disallowed (%)

0.13