Table 1 CryoEM data collection, refinement, and validation statistics
TssKSNFG (EMDB-9341) (PDB 6N38) | TssKSN (EMDB-9342) | TssFG (EMDB-9343) | |
---|---|---|---|
Data collection and processing | |||
Magnification | 36,496 | 36,496 | 36,496 |
Voltage (kV) | 300 | 300 | 300 |
Electron exposure (e–/Å2) | 40 | 40 | 40 |
Defocus range (μm) | 0.2–4.0 | 0.2-4.0 | 0.2-4.0 |
Pixel size (Å) | 1.37 | 1.37 | 1.37 |
Symmetry imposed | |||
Initial particle images (no.) | 369,971 | 369,971 | 369,971 |
Final particle images (no.) | 57,567 | 57,567 | 57,567 |
Map resolution (Å) | 3.7 | 3.6 | 3.9 |
FSC threshold | 0.143 | 0.143 | 0.143 |
Map resolution range (Å) | |||
Refinement | |||
Model resolution (Å) | 3.7 | ||
FSC threshold | 0.5 | ||
Model resolution range (Å) | |||
Map sharpening B factor (Å2) | −69 | ||
Model composition | |||
Non-hydrogen atoms | 24,934 | ||
Protein residues | 3145 | ||
Ligands | |||
R.m.s. deviations | |||
Bond lengths (Å) | 0.013 | ||
Bond angles (°) | 1.286 | ||
Validation | |||
MolProbity score | 0.95 | ||
Clashscore | 1.02 | ||
Poor rotamers (%) | 0.07 | ||
Ramachandran plot | |||
Favored (%) | 97.20 | ||
Allowed (%) | 2.67 | ||
Disallowed (%) | 0.13 |