Fig. 1
From: BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitment
DNA:RNA hybrids form at DSBs independently of the genomic context. a Schematic representation of DNA:RNA hybrids (in red) that can be generated upon the hybridization of mRNA (top) or dilncRNAs (bottom) with resected DNA ends at the I-PpoI cut site within DAB1 gene. b DRIP-qPCR analysis at the I-PpoI cut site within a genic (DAB1 gene) or c nongenic locus in HeLa cells transfected with the I-PpoI nuclease. d DRIP-qPCR analysis at a nongenic AsiSI cut site in DIvA cells. Bar graphs in b, c and d show fold induction of DNA:RNA hybrid levels in cut samples relative to uncut. RNase H treatment was performed on cut samples to demonstrate specificity of the signal. Error bars represent s.e.m. (n ≥ 3 independent experiments). *P < 0.05, **P < 0.01 (two-tailed Student’s t test). e Boxplot representing log2 ratio of the fold change of DNA:RNA hybrid reads in cut compared to uncut samples at the BLISS detected top 50 cut AsiSI sites at different distances from the DSBs. *P < 0.05, **P < 0.01 (Wilcoxon signed-rank test). Source data are provided as a Source Data file
