Fig. 3

dilncRNAs contribute to HR proteins recruitment to DSBs and HR-mediated repair. a Representative images of DNA-end resection markers: ssDNA (visualized by BrdU native staining), RPA2, and RPA2 pS4/8 foci, co-stained with cyclin A, as S/G2-phase marker, in irradiated (5 Gy) HeLa cells treated with H₂O or α-amanitin. Scale bar: 10 μm. b Dot plots show the number of signals/foci in a. At least n = 60 cells were counted from three independent experiments. Lines represent mean ± s.e.m. c Representative images of BRCA1, BRCA2, and RAD51 foci co-stained with cyclin A or CENPF, as S/G2-phase markers, in irradiated (5 Gy) HeLa cells treated with H₂O or α-amanitin. Scale bar: 10 μm. d Dot plots show the number of foci in c. At least n = 120 cells were counted from three independent experiments. Lines represent mean ± s.e.m. e–g DR-GFP cells are treated with control ASO (CTRL), ASOs matching dilncRNAs (A/B), or inactive ASOs. e HR efficiency is monitored by FACS analysis of the percentage of GFP-positive cells or f by genomic semiquantitative PCR with primers P1 and P2 that only amplify the recombined GFP sequence generated after HR (see Supplementary Fig. 4a). g SSA efficiency is assessed by monitoring the 0.8 kb amplicon generated by genomic semiquantitative PCR with primers F1 and R2 (see Supplementary Fig. 4a). Bar graphs show the mean of n ≥ 2 independent experiments. Error bars represent s.e.m. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ^****P < 0.0001 (two-tailed Student’s t test). Source data are provided as a Source Data file