Fig. 2

Abnormal repartition of monocyte subsets in Mir150^−/− (KO) mice. a, b Representative flow cytometry scatter plot of CD11b⁺ monocytes (a) or CD43⁺ monocytes (b) gated on Ly6C expression in bone marrow of a wild-type (WT) and a Mir150^−/− mouse. c Flow cytometry quantification of Ly6C^high and Ly6C^low monocyte subsets in the bone marrow of WT and Mir150^−/− (KO) animals (12/group) expressed as a fraction of total monocytes (upper panels) or absolute number per mouse femur (lower panels). Mean ± SEM, two-tailed unpaired t test: **P < 0.01; ****P < 0.0001. d, e. Representative flow cytometry scatter plot of CD11b⁺ monocytes (d) or CD43⁺ monocytes (e) gated on Ly6C expression in the peripheral blood of a WT and Mir150^−/− mouse. f Flow cytometry quantification of Ly6C^high and Ly6C^low monocyte subsets in the peripheral blood of young (<6 months, WT = 28, KO = 17) or older (6–26 months, WT = 26; KO = 21 KO) animals expressed as fraction of total monocytes (upper panels) or absolute number per mouse (lower panels). Mean ± SEM, two-tailed unpaired t test: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. g Flow cytometry measurement of the fraction of Annexin V⁺ cells among Ly6C^high and Ly6C^low monocytes in the peripheral blood and bone marrow of 6 WT (gray bars) and 10 Mir150^−/− (red bars) animals (mean ± SEM). NS nonsignificant