Fig. 6
From: A miR-150/TET3 pathway regulates the generation of mouse and human non-classical monocyte subset
Methylation of MIR150 region 3 in CMML classical monocytes. a DNA methylation of MIR150 region 3 (R3) was analyzed in sorted CD14+ peripheral blood monocytes from six young (<65 years old) and 8 older (>65 years old) healthy donors and 10 CMML patients. White ball: no methylation; gray ball: methylated on one strand; black ball: methylated on two strands. b DNA methylation status of R3 was analyzed in sorted CD14+ monocytes from 12 CMML patients before (+0) and after (+Dac) decitabine treatment, including five responders and seven non-responders. White ball: no methylation; gray ball: methylated on one strand; black ball: methylated on two strands. c–e qRT-PCR analysis of FCGRT and hsa-miR-150 expression in sorted cells collected from 12 healthy donors (CTL) and 10 CMML patients. Samples are those studied by bisulfite sequencing. FCGRT gene expression is normalized to RPL32 in sorted monocytes (c), hsa-miR-150 expression is normalized to RNU-44 (d) in sorted monocytes and in CD3+ T lymphocytes (e). Mean ± SEM, unpaired t test: *P < 0.05. NS, nonsignificant
