Fig. 3

Survival of iAs^V-exposed humanized As3mt-KO mice. Groups of As3mt-KO mice (i.e., two or three replicate cages containing between two and five mice each) received a fecal transplant from a healthy human donor (humanized) either 10 days prior to iAs^V exposure (F0) or at birth from a humanized dam (F1). a Survival of F0 and F1 groups was not significantly different compared to conventional, Sham-treated As3mt-KO mice following exposure to 100 ppm iAs^V (F0, p = 0.6500; F1, p = 0.1887; Mantel–Cox test), but survival in both groups was significantly greater than GF As3mt-KO (p < 0.0001, Mantel–Cox test). Five groups of humanized As3mt-KO mice, each representing a unique human microbiome donor, were exposed to 100 ppm iAs^V (two to four replicate cages per group). b Median survival in all donor groups was significantly greater than that of GF As3mt-KO mice (p ≤ 0.0014, pairwise Mantel–Cox), and equivalent to or greater than that of Sham-treated conventional mice. There were also significant differences in survival among different humanized groups (p = 0.0030, Mantel–Cox). Pairwise comparisons (Mantel–Cox test) are shown in Supplementary Table 4. Survival of GF and conventional, Sham-treated As3mt-KO from previous figures (Fig. 2c and b, respectively) are shown in both panels for comparison