Fig. 6
From: Distinct adaptive mechanisms drive recovery from aneuploidy caused by loss of the Ulp2 SUMO protease
Mutations in Ubc9, Uba2, and Aos1 suppress ulp2Δ aneuploidy and other defects. a Plasmid eviction strategy to test ubc9, uba2, and uba2 aos1 mutants that were also ulp2Δ. b, f, j Growth assays of the indicated strains. After spotting cells in fivefold serial dilutions, SD–Leu plates were incubated for 2 days at 30 °C. c, g, k Flow cytometry analysis of DNA content of the indicated strains. d, h, l Immunoblot analysis of SUMO conjugates in whole-cell lysates. e, i, m qPCR ploidy assays. Error bars indicate the SD calculated from two independent experiments
