Fig. 2
Molecular assembly of an HIV-1 Env lattice with improved diffraction. a The asymmetric unit of the P63 lattice is shown with the PGT122 Fab colored in cyan (HC; heavy chain) and light cyan (LC; light chain); 35O22 colored in magenta (HC; heavy chain) and light magenta (LC; light chain). gp120 and gp41 of BG505 SOSIP.664 Env trimer are colored as black and gray respectively. b Crystal packing within the P63 lattice, highlighting the lack of crystal contacts between Env trimers (top right). c Maps and diffraction statistics before and after lattice engineering. 2Fo-Fc electron density maps of the BMS-378806 binding pocket, shown as blue mesh, are contoured at 2σ. (*The original data were truncated with different criteria. For fair comparison, the structure factor was downloaded from the Protein DataBank and the same criteria applied for truncation.) d Summary of the best changes identified in the antibody fragments to improve the diffraction
