Fig. 1

Ezh2 is required for T_FH and B cell responses to viral infection. a–f T cell-specific ablation of Ezh2 affects T_FH and B cell responses to viral infection. WT and Ezh2^–/– mice were infected with VacV. On 8 dpi, CD44^hiCD62L^– activated CD4⁺ T cells were detected in the spleen (a), and further analyzed for CXCR5⁺SLAM^lo T_FH cells and CXCR5 expression levels (b), CXCR5⁺PD-1^lo T_FH and CXCR5⁺PD-1^hi GC-T_FH cells (c), and CXCR5⁺Bcl6⁺ T_FH cells (d). Splenic B220⁺CD19⁺ cells were analyzed for Fas⁺GL7⁺ GC B cells (e) and CD138⁺IgD^lo plasma cells (f). Contour plots are representative of two experiments, and cumulative data on frequency and numbers of each subset are the means ± s.d., and each dot represents a mouse. (g) Detection of VacV-specific IgG in sera of infected mice collected on 8 dpi (n = 4 for WT and 5 for Ezh2^–/–). *p < 0.05; **p < 0.01; ***p < 0.001 by unpaired two-tailed Student’s t-test