Fig. 3
Glucose incorporation into nucleotides is altered upon PHGDH inhibition. a Schematic of [U-13C] glucose labeling of purines. b Mass-isotopomer distribution (MID) of IMP (inosine monophosphate) from [U-13C] glucose in cells treated with 25 μM WQ-2101. Data are the mean of three biological replicates, and error bars represent s.e.m. P < 0.05 [*], P < 0.01 [**], Student’s t-test. c Schematic of [U-13C] glucose labeling of pyrimidines. d Mass-isotopomer distribution (MID) of UMP (uridine monophosphate) from [U-13C] glucose in cells treated with 25 μM WQ-2101. Data are the mean of three biological replicates, and error bars represent s.e.m. P < 0.05 [*], P < 0.01 [**], P < 0.005 [***], Student’s t-test. For labeling experiments, cells were pre-treated with inhibitors for 4 h followed by introduction of [U-13C] glucose-containing medium including inhibitors for 20 h
