Fig. 2

Biological incorporation of fluorescent-tagged glucose (6CF-Glc) into BC fibers. a, b Images of 6CF-BC fermentation under standard conditions (30 °C) on days 2, 3, 4, and 5. The thickness of a HC-6CF-BC (high concentration of 6CF-Glc, 0.95 mg mL⁻¹) was thinner than b LC-6CF-BC (low concentration of 6CF-Glc, 0.38 mg mL⁻¹). The scale bar corresponds to 1 cm. c–e Images of 6CF-BC, BC, and Im-6CF/BC observed under UV light (365 nm). c 6CF-BC showed green fluorescence, whereas d Im-6CF/BC and e BC showed no fluorescence. The scale bar corresponds to 2 cm. f–i Images of 6CF-BC, BC, and Im-6CF/BC observed with CLSM (excitation wavelength 488 nm). f HC-6CF-BC and g LC-6CF-BC showed green fluorescence, whereas h Im-6CF/BC and i BC showed no fluorescence. The scale bar corresponds to 100 μm