Fig. 5

Prmt5 is necessary for GC expansion. a–l Cγ1-cre (Ctrl) versus Prmt5^F/F Cγ1-cre (F/F) mice. a Representative flow cytometry plots for GC B cells at various times post-SRBC immunization. Mean ± s.d. absolute GC B cell numbers from n mice from two to three experiments are plotted. b GCs per spleen section in individual mice (symbols), with means (bars). c Representative fluorescent microscopy images of spleen sections from mice in a stained for B cells (B220), T cells (CD3), FDCs (CD35), and activated B cells (GL7). Scale bar, 100 µm. d Representative IF confocal images in splenic sections from immunized mice, stained for the indicated markers, Prmt5 and sDMA (SYM11). GCs are contoured. Scale bars, 100 µm. e Representative histograms of activated pan-caspase staining in splenic GC B cells at day 8 post-SRBC immunization. Etoposide (3 µM) was used to induce apoptosis as a positive control. Mean + s.d. proportion of caspase⁺ GC B cells for six mice per group from two independent experiments are plotted. f Representative histograms of Ki67 expression in splenic GC B cells. Mean + s.d. proportion of Ki67^low GC B cells for individual mice (symbols) from two experiments and means (bars) are plotted. g Representative flow cytometry plots of dark zone (DZ), light zone (LZ), and a CXCR4⁻ CD86^low GC B cells (gated on B220⁺ Gl7⁺ CD95⁺) in SRBC-immunized mice. The proportion of CXCR4⁻ CD86^low GC B cells for individual mice (symbols) from two to three experiments and means (bars) are plotted. h GC B cells in mice infected with H. polygyrus for 14 days analyzed as in g. Data from two experiments. i Representative histograms of Ki67 levels in GC B cell subsets in mice immunized with SRBC. j GC B cells and subpopulations as in g for Aicda^−/− Cγ1-cre (Ctrl) or Aicda^−/− Prmt5^F/F Cγ1-cre (F/F) mice immunized with SRBC. k Histograms of AID-GFP levels for individual Aicda-GFPtg Cγ1-cre (Ctrl) or Aicda-GFPtg Prmt5^F/F Cγ1-cre (F/F) mice at day 9 post-SRBC immunization. The AID^dim/AID^high ratio for individual mice (symbols) from two experiments and means (bars) are plotted. p-Values indicated throughout are by an unpaired, two-tailed Student's t test