Table 1 Binding affinities of different histone peptides to the ePHD6 of MLL3 and MLL4 measured by ITC

From: Structural insights into trans-histone regulation of H3K4 methylation by unique histone H4 binding of MLL3/4

Histone peptides

Kd (μM)

MLL3_(ePHD6)1055–1144

MLL4_(ePHD6)1475–1564

H41–24

23 ± 3

13 ± 1

H41–12

NB

NB

H41–12R3me2a

NB

NB

H411–21

35 ± 2

52 ± 2

H41–24R3A

66 ± 3

28 ± 1

H31–20

NB

NB

H31–15K4me2

NB

NB

H31–15K4me3

NB

NB

  1. Kd values were calculated from single measurement and errors were estimated from fitting curve by Origin software package
  2. NB, no detectable binding
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