Fig. 2

iNKT and γδ-T cells are armed for a competent IL-23 response. a RORC and IL23R mRNA transcripts expressed in iNKT, TCRγδ-hi/int, and CD161+ conventional T cells as measured by PrimeFlow technology (n = 3). Gray histograms represent FMO. Quantitative data are presented in Supplementary Fig. 2B. b PBMC (left) or sorted γδ-T cells were cultured with αGalCer (PBMC, n = 18) or aCD3/aCD28 Abs (γδ-T cells, n = 6), in the presence of IL-2 or IL-2 supplemented with IL-23, IL1β, TGFβ1 to induce IL-17 cytokine response as measured from viable iNKT (6B11+ TCRvβ11 + CD3+ cells) or γδ-T cells (TCRγδ+ CD3+). Flow data from one representative experiment is shown. c, d Quantitative overview of cytokine production measured in γδ-T cell (C; IL-2: black symbols; IL-23: red symbols) and iNKT (D, IL-2 condition: black symbols; IL-23: red symbols) cultures as described in b (IL-23 vs. IL-2 condition, *p < 0.05, **p < 0.01 determined by paired t-tests). e FACS analyses were performed on gated IL17+ and IL17− iNKT cells depicted in experiments described in b (n = 6–8). *p < 0.05, **p < 0.01 determined by paired t-tests (IL-17- cells: black bars; IL-17+: white bars). f qPCR assays on selected target genes, were performed on IL17+ and IL-17− iNKT cells isolated by means of a IL-17 Capture Assay (qPCR, n = 3, Mann-Whitney test). (IL-17- cells: black bars; IL-17+: white bars). Data throughout this figure are presented as mean ± SEM