Fig. 6
Molecular and spatial variants in Vrs1. a Gene structures (exon: red bar; intro: yellow bar; UTR: blue bar) of Vrs1 with the relative positions of the SNPs (triangle) and INDELs (rhombus), respectively. The golden triangle or rhombus indicates the same genotype compared with the reference gene sequences (Vrs1.b3: gi|119943316) and the lime green indicates difference. The vrs1.a4 alleles differ from other domesticated Vrs1 alleles by a unique SNP (G/T) in position 387 of the Vrs1.b3 sequences. b Median-joining networks Vrs1 haplotypes of barley accessions based on Vrs1 SNPs. The INDELs were considered as SNPs for distinguishing b2 and a2. Hsp H. spontaneum, Hag H. agriocrithon, Hgy H. var. gymnospermum Korn, TWB Tibetan weedy barley. Three two-rowed qingke cultivar accessions which mixed with pedigree of two-rowed barley, carried the allele Vrs1.b3. c Degree of haplotype diversity around the Vrs1 locus in the barley genome. The golden region of corresponding accessions indicates the same genotype compared with the barley genome (Morex: Vrs1.a1); and the green region indicates difference. d Haplotype distributions according to geography for Vrs1. The six Vrs1.b2 accessions were not shown on the map because of the absence of clear geographical location. Uzbekistan highlighted by blue ellipse was the possible origin area of Vrs1.a4 reported by Pourkheirandish et al.11. d was generated in R (V3.4.3) using packages maps, maptools, plyr, ggrepel, and ggplot2. Geographic information data were obtained from Global Administrative Areas database (GADM V2.8, November 2015). Source data are provided as a Source Data file
