Fig. 5 | Nature Communications

Fig. 5

From: BRCA2 deficiency instigates cGAS-mediated inflammatory signaling and confers sensitivity to tumor necrosis factor-alpha-mediated cytotoxicity

Fig. 5

Proteomic and transcriptomic analysis reveals upregulation of pro-inflammatory genes upon BRCA2 depletion. a Workflow of SILAC-MS analysis of BT-549 and HCC38 cell lines with indicated shRNAs. b Log2 ratios (heavy vs light) of proteins that were measured in at least three out of four independent MS analyses in BT-549 (left panel) or HCC38 (right panel) cells. Black dots represent the mean of log2 ratios from three or four experiments. c ENRICHR was used to analyze pathway enrichment in top 25 upregulated proteins in response to BRCA2 depletion in BT-549 cells and HCC38 cells. The top 10 enriched Reactome datasets are displayed. d, e RNA sequencing was performed on BT-549 and HCC38 cells harboring shLUC or shBRCA2 #2, treated for 72 h with or without doxycycline. Gene set enrichment analysis (GSEA) using ‘Hallmark’ gene sets showed enrichment of Interferon Gamma response (d) and TNFA signaling via NF-κB (e) in BRCA2-depleted cells. f, g Top 10 enriched Hallmark gene sets in BRCA2-depleted BT-549 (f) and HCC38 (g) cells compared to control cell lines. The top 10 list of enriched pathways can be found in Supplementary Fig. 7a

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