Fig. 6

Endothelial hierarchy RNA sequencing and gene expression analysis. a, b Principal component analysis and hierarchical clustering demonstrating the distinct clustering of each population segregated from each other (n = 5). c Differentially expressed genes (*p < 0.05 after multiple testing correction) were identified between EVP and D populations. Differentiated endothelial markers Pecam, Kdr, and Nos3 were all significantly upregulated in D compared to EVP (***p < 0.001 vs EVP). Results presented as mean ± SEM. EVP endovascular progenitor, D definitive differentiated