Fig. 4
From: Molecular recognition of the native HIV-1 MPER revealed by STED microscopy of single virions
Differential binding of anti-MPER antibodies 10E8 and 4E10 to virions as revealed by quantitative STED microscopy. a Representative images of virions (Vpr.GFP, green) and antibodies (KK114, red) (top) and measured KK114 intensities (bottom) for 10E8 WT (n = 776), 10E8 WG (n = 1375), 4E10 WT (n = 644), and 4E10 Δloop (n = 1109). Each point in the bottom plot represents the KK114 intensity sum measured in a single viral particle. Negative samples (n = 795) were measured in the absence of labeled antibody. Data from a single experiment. b 10E8 and 4E10 STED signals measured in Env(-) (n = 223 and 202), EnvNL4-3 (n = 3336 and 3165), and EnvJR-CSF (n = 1664 and 1303) HIV-1 virions. For the matter of comparison signals from single virions from two or more independent experiments have been normalized to 10E8-EnvNL4-3 signal after background subtraction. Histograms displaying F/v frequencies measured for both antibodies (50 ng μL−1) in NL4-3 (c) and JR-CSF (d) viruses. The statistical significance was assessed by Kruskal–Wallis one-way analysis of variance. If not noted otherwise, differences were not significant at the 0.05 level. In a and b results are shown in box-plots (center line, median; square, mean; box, IQR; whiskers, upper and lower inner fences). Scale bars are 100 nm
