Fig. 3
From: Promotion of homology-directed DNA repair by polyamines
Polyamines stimulate RAD51-mediated DNA strand exchange. a The effects of DFMO (500 µM) treatment and RAD51 overexpression on HR efficiency were examined in U2OS cells using the DR-GFP reporter assay. b Schematic of the DNA strand exchange assay. The asterisk denotes the 32P-label. c–e Putrescine in c, Spermidine in d, or Spermine in e enhances the activity of RAD51-mediated DNA strand exchange in a concentration-dependent manner. Note that the ATP was required for strand exchange regardless of whether polyamine was present or not. The reaction time was 20 min. To normalize the percentage of product, each signal was subtracted from the percentage of product in control reaction (lane 1). f The effects of all three polyamines on RAD51-mediated DNA strand exchange were examined. The reaction time was 20 min. ***P < 0.001; ****P < 0.0001. Data are the mean ± s.e.m. from three independent experiments (n = 3). Statistics was performed by one-way ANOVA with Tukey’s post hoc test. Source data are provided as a Source Data file
