Fig. 1

Upregulation of ZFP36L1 in chondrocytes stimulated with OA-associated catabolic regulators. a Microarray analysis of AU-rich element (ARE)-binding proteins in chondrocytes treated with IL-1β or infected with Ad-HIF-2α or Ad-ZIP8. b, c qRT-PCR analyses of ZFP36 family members in chondrocytes treated with IL-1β or infected with 800 MOI of control virus (Ad-C) or the indicated MOIs of Ad-HIF-2α or Ad-ZIP8 (n = 3 or 5). d Representative images of Alcian blue staining and ZFP36L1 immunostaining in undamaged and damaged regions of OA cartilage from the same patient (n = 7 patients). e Representative images of Safranin-O staining and ZFP36L1 immunostaining in mouse OA cartilage caused by DMM surgery (n = 5 mice per group) or by IA injection of Ad-HIF-2α or Ad-ZIP8 (n = 5 mice per group). Means ± s.e.m. with one-way ANOVA (*P < 0.05, **P < 0.005, and ***P < 0.0005). Scale bar: 50 μm