Fig. 2
Overexpression of ZFP36L1 in joint tissues does not modulate OA pathogenesis. a, b WT mice were IA injected with Ad-C (n = 6 mice) as a control or Ad-ZFP36L1 (n = 8 mice). Representative immunostaining images of ZFP36L1, MMP3, and MMP13 in cartilage sections (a). Cartilage destruction and synovitis were determined by Safranin-O staining and H&E staining 3 weeks after IA injection (b). c Protein and mRNA levels of the indicated molecules in chondrocytes infected with Ad-C (800 MOI) or the indicated MOIs of Ad-ZFP36L1 for 36 h. Representative images are presented from six independent primary cultures of chondrocytes. d, e Sham-operated (n = 4 mice) or DMM-operated (n = 7 mice) WT mice were IA injected with Ad-C as a control or Ad-ZFP36L1 to overexpress ZFP36L1 in joint tissues. Representative Safranin-O staining images of joint sections (d) and scoring of OARSI grade, osteophyte maturity, and subchondral bone plate thickness (e) at 8 weeks after operations. Means ± 95% CI with Mann–Whitney U test for OARSI grade and osteophyte maturity. Means ± s.e.m. with two-tailed t-test for SBP thickness. Scale bar: 50 μm
