Fig. 6 | Nature Communications

Fig. 6

From: GORAB scaffolds COPI at the trans-Golgi for efficient enzyme recycling and correct protein glycosylation

Fig. 6

Scyl1-dependent recruitment of COPI to artificial membranes. a Liposome recruitment assay with purified MBP-Scyl1, myr-Arf1 and recombinant coatomer. Inputs (2%) and membrane-bound fractions (40%) were subjected to SDS-PAGE and blotted for MBP, ε-COP and Arf1. b Liposome recruitment assay with purified MBP-IPIP27A (as negative control), MBP-Scyl1 and myr-Arf1 and recombinant coatomer. Inputs (2%) and membrane-bound fractions (40%) were subjected to SDS-PAGE and blotted for MBP, δ-COP, ε-COP and Arf1. c Quantification of recruitment of Arf1 and coatomer (δ-COP, ε-COP) to liposomes in the presence of MBP-IPIP27A and MBP-Scyl1. Error bars represent mean ± SD, n = 6 independent experiments, **p < 0.01, ***p < 0.001, unpaired t-test

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