Fig. 1 | Nature Communications

Fig. 1

From: Intestinal epithelial N-acylphosphatidylethanolamine phospholipase D links dietary fat to metabolic adaptations in obesity and steatosis

Fig. 1

Validation of IEC Napepld deletion. a Napepld mRNA expression in the jejunum, colon, liver and epididymal adipose tissue (EAT) in ND-fed WT and Napepld∆IEC mice (n = 8–10). b NAPE-PLD protein levels in the colon. Representative western-blot of NAPE-PLD and β-Actin (n = 9–10). c Levels of N-acylethanolamines and 2-acylglycerols in small intestinal epithelial cells of ND-fed WT and Napepld∆IEC mice (n = 7–9) were determined by using high-performance liquid chromatography-MS using an LTQ Orbitrap mass spectrometer as described in the methods. Dark blue: WT ND mice, light blue: Napepld∆IEC ND mice. Data are presented as the mean ± s.e.m. *, ** and *** indicate a significant difference versus WT ND (Respectively P < 0.05, P < 0.01 and P < 0.001) according to Mann–Whitney test

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