Fig. 4

Exacerbation of HFD-induced obesity in Napepld^∆IEC mice. a Body weight (g) over an 8 weeks period. b Fat mass gain (g) over an 8 weeks period. c Weight of different white adipose tissue depots and BAT (g). d Daily food intake (Kcal/day) measured in metabolic chambers during indirect calorimetry studies at the 8th week of HFD feeding. e Energy measured in the feces at the 8th week of HFD feeding (Kcal g feces⁻¹). f Light and dark cycle energy expenditure (Kcal h⁻¹ Kg body weight⁻¹) measured in metabolic chambers. g Light and dark cycle of CO₂ production (l h⁻¹ Kg body weight⁻¹). h Light and dark cycle of O₂ consumption (l h⁻¹ kg body weight⁻¹). Data in a–c correspond to the results of three independent experiments. For a–c, n = 26–28. For d, f–h, n = 5. For e, n = 4–6. Dark blue: WT ND mice, light blue: Napepld^∆IEC ND mice, purple: WT HFD mice and pink: Napepld^∆IEC HFD mice. Data are presented as the mean ± s.e.m. Data with different superscript letters are significantly different (P < 0.05) according to repeated measures two-way ANOVA followed by Tukey’s post-hoc test (a, b) or regular two-way ANOVA followed by Tukey’s post-hoc test (c, h)