Fig. 5

Phenotype of Napepld^∆IEC mice after 16weeks is associated with lower energy expenditure. a Fat mass gain (g) after a 16 weeks period. b Daily HFD intake (Kcal/day) measured in metabolic chambers during indirect calorimetry studies at the 16th week of HFD feeding. c Light and dark cycle energy expenditure (Kcal h⁻¹ Kg body weight⁻¹) measured in metabolic chambers. d Light and dark cycle of CO₂ production (l h⁻¹ Kg body weight⁻¹). e Light and dark cycle of O₂ consumption (l h⁻¹ kg body weight⁻¹). f Light and dark cycle spontaneous locomotor activities (Ambulatory Counts h⁻¹). g mRNA expression of Elovl3 and Ucp1 in the subcutaneous adipose tissue. For a–f, n = 8–10. For g, n = 8–10. Purple: WT HFD mice and pink: Napepld^∆IEC HFD mice. Data are presented as the mean ± s.e.m. Asterisks (* and **) indicate a significant difference versus WT HFD (Respectively P < 0.05 and P < 0.01) according to Mann–Whitney test or repeated measures two-way ANOVA followed by Bonferroni’s post-hoc test