Fig. 6
IEC-specific Napepld exacerbates diet-induced liver steatosis. a Liver weight (g). b Liver lipid content measured by gravimetry (as percentage of liver weight). c Representative liver Oil red O staining (scale bar: 100 µm) and mean lipid droplet size (µm², n = 5–7). d Liver triglycerides (nmol mg−1). e Liver cholesterol (nmol mg−1). f Liver thiobarbituric acid-reactive species (TBARS, pmol mg protein−1). g Plasma alanine aminotransferase (ALT) activity measured in the cava vein (IU l−1) (n = 6–8). h Plasma aspartate aminotransferase (AST) activity measured in the cava vein (IU l−1) (n = 6–8). i Histological fibrosis analysis using Sirius red staining expressed in % of area stained (n = 7–8). j mRNA expression of Fatp4, Mttp, and Cd36 in the duodenum (n = 9–12). k mRNA expression of Fatp4, Cd36, and Mttp in the liver (n = 7–8). l mRNA expression of Flt4, Dll4, Lyve1, Fabp1, Npc1l1, Ldlr, Apob, Apoa4 and Apoe in the duodenum (n = 9–12). m Plasma TG in the caudal vein after a lipid load challenge (300 μl of Intralipid 20% emulsion) (mMol), Inset: area under the curve (AUC) of TG level evolution during lipid load test (n = 7–12). n Plasma NEFA in the caudal vein after a lipid load challenge (300 μl of Intralipid 20% emulsion) (mMol), Inset: area under the curve (AUC) of NEFA level evolution during lipid load test (n = 7–12). o Gastrocnemius TG content (nmol mg of tissue−1) (n = 7–8). n = 26–28 for a, b, and d–f. Data a, b, and d–f correspond to the results of three independent experiments. Dark blue: WT ND mice, light blue: Napepld∆IEC ND mice, purple: WT HFD mice and pink: Napepld∆IEC HFD mice. Data are presented as the mean ± s.e.m. Data with different superscript letters are significantly different (P < 0.05) according to regular two-way ANOVA followed by Tukey’s post-hoc test. Asterisk (*) indicates a significant difference versus WT HFD (P < 0.05) according repeated measures two-way ANOVA followed by Bonferroni’s post-hoc test
