Fig. 7
From: Mature IgDlow/- B cells maintain tolerance by promoting regulatory T cell homeostasis
Following adoptive transfer a subset of BDL are stable phenotypically. C57BL/6 CD45.1 FO (a, b) or BDL (c, d were FACS purified, CFSE labeled, and adoptively transferred (1.5 × 106) into C57BL/6J CD45.2 mice that were sublethally irradiated (500 rad) 11 days prior. On days 1 and 2 the presence of FO, BDL, and MZ B cells was assessed in the spleen. The transferred cells were gated as CD45.1+ and the percentage of each B cell subset within the gate is provided and shown as dot plots with CFSE. The inset is a histogram of the CFSE data with the percentage of cells having undergone proliferation indicated. Data shown are one representative experiment of four
