Fig. 1
From: Lin28 and let-7 regulate the timing of cessation of murine nephrogenesis
Expression of Lin28 and let-7 in mouse embryonic kidney. a Western blot analysis against Lin28a, Lin28b, Six2, and α/β-Tubulin proteins in lysates collected from dissected embryonic day 12.5–18.5 (E12.5–E18.5), newborn (NB), postnatal day 2 (P2), and adult wild-type kidneys. b Absolute qRT-PCR analysis measuring the levels of Lin28a and Lin28b mRNAs in wild-type kidneys. n = 3 for each sample type. c, d Relative qRT–PCR analysis measuring the levels of mature and precursor (pre-) let-7 miRNAs in wild-type kidneys. n = 3–5 for each sample type. Error bars represent mean ± SD. (*) = p < 0.05, unpaired, two-tailed Student’s t test
