Fig. 2

GNPTAB is required for efficient EBOV infection. a GNPTAB⁻ cells survive EBOV infection. Parental HAP1, GNPTAB⁻, or NPC1⁻ knockout cells were infected with wild-type EBOV at an MOI of 0.3, and viability relative to mock-infected cells was determined 6 days p.i. Data represent the mean ± s.d. of three independent experiments. Statistical analysis was performed with a two-tailed Student’s t-test, with significance shown as ***, P = 0.0005. b Infection with the EBOV-ZsG reporter virus is reduced in GNPTAB⁻ knockout cells. Parental HAP1 (orange circles), GNPTAB⁻ knockout (blue squares) and NPC1⁻ knockout (black triangles) cells were infected with EBOV-ZsG at an MOI of 0.1 and ZsG fluorescence was measured over time. Data represent the mean ± s.d. of 4 biological replicates. A representative of three independent experiments is shown. c Wild-type EBOV growth is impaired in GNPTAB⁻ knockout cells. Parental and knockout HAP1 cells were infected with wild-type EBOV at an MOI of 0.5. Samples of culture supernatant were taken at intervals and the EBOV titer was determined. Data represent the mean ± s.d. of three biological replicates. A representative of two independent experiments is shown