Table 1 Selected hits from the CRISPR-Cas9 screen for genes important for authentic EBOV infection
Gene identifier | P-value | No. of enriched sgRNAs | Name | Comment |
|---|---|---|---|---|
NPC1 | 2.83E−07 | 5 | NPC Intracellular Cholesterol Transporter 1 | EBOV receptor |
SPNS1 | 2.83E−07 | 4 | Sphinholipid Transporter 1 (Putative). | Putative lysosomal transporter protein |
SLC30A1 | 1.42E−06 | 4 | Solute Carrier Family 30 Member 1 | Zinc transporter protein 1 |
VPS16 | 3.12E−04 | 3 | Vacuolar Protein Sorting 16 Homolog | HOPS complex subunit |
KLHDC3 | 1.94E−04 | 3 | Kelch Domain Containing 3 | |
STARD13 | 1.94E−04 | 3 | StAR Related Lipid Transfer (START) Domain Containing 13 | GTPase-activating protein |
GNPTAB | 7.17E−04 | 3 | N-Acetylglucosamine-1-Phosphate Transferase Subunits Alpha and Beta | Catalyzes synthesis of mannose 6-phosphate on newly synthesized lysosomal enzymes |
ZNF646 | 1.14E−03 | 2 | Zinc Finger Protein 646 | |
VPS33A | 9.60E−04 | 2 | Vacuolar Protein Sorting 33 Homolog A | HOPS complex subunit |
UVRAG | 1.15E−03 | 2 | UV radiation resistance associated. | Required for Influenza A and VSV entry32 |
VPS18 | 9.01E−03 | 2 | Vacuolar Protein Sorting 18 Homolog | HOPS complex subunit |
CTSL | 5.82E−03 | 1 | Cathepsin L | |
CTSB | 5.95E−02 | 1 | Cathepsin B |
