Fig. 5
Sequencing of mRNA and rRNA 3′ extensions in KPAF4 RNAi background. a Length distribution of short mRNA and 12S rRNA tails. Non-encoded 3′ end extensions (MiSeq instrument, Illumina, single biological replicate) were individually binned into 10-nt length groups. Mock-induced and RNAi datasets, indicated by blue and red bars, respectively, represent percentage of the total number of reads. b Length distribution of long mRNA tails. Non-encoded 3′ end extensions (PacBio RS II instrument, two biological replicates) were individually binned into 10-nt length groups before 100 nt, and in 50-nt groups thereafter. Mock-induced and RNAi datasets are indicated by blue and red bars, respectively, that represent percentage of the total number of reads. c Positional nucleotide frequencies in short mRNAs and 12S rRNA tails. A nucleotide percentage was calculated for each position that contained at least 5% of the total extracted sequences. The nucleotide bases are color-coded as indicated. Arrows show positions of equal adenosine and uridine frequencies. d Positional nucleotide frequencies in long mRNA tails. A nucleotide percentage was calculated for each position that contained at least 5% of the total extracted sequences. The nucleotide bases are color-coded as indicated. Arrows show positions of equal adenosine and uridine frequencies
