Fig. 2

Apyrase release by E. coli. a Schematic model of apyrase release by E. coli (E.S., extracellular space; O.M., outer membrane; P.S., periplasmic space; I.M., inner membrane; O.M.V., outer membrane vesicle). b Apyrase activity in culture supernatants of the indicated E. coli transformants at different OD₆₀₀ expressed as percentage of non-degraded ATP. c Apyrase activity in OMVs isolated from E. coli^pBAD28 and E. coli^pApyr. d ATP concentrations in culture medium (bars) and bacterial growth (OD₆₀₀) over time for E. coli^pBAD28 (left panel) or E. coli^pApyr (right panel). L-arabinose was added at time 0. One representative experiment out of at least three is shown