Fig. 3
BhCas12b v4 mediates genome editing in human cells. a Indel activity in 293T cells of AsCas12a at 28 TTTV targets and BhCas12b v4 at 33 ATTN targets. Each dot represents a single target site, averaged from n = 4 replicates. b Average indel length during genome editing with 30 active BhCas12b guides, 45 active AsCas12a guides, and 39 active SpCas9 guides. c Schematic of a DNMT1 region targetable by SpCas9 and Cas12a/b nucleases and a 120-nt ssODN donor containing a TG-to-CA mutation and PAM-disrupting mutations. d Indel activity of each nuclease at the DNMT1 locus. Error bars represent s.d. from n = 8 replicates. e Frequency of homology-directed repair (HDR) using a target strand (T) or non-target strand (NT) donor. Gray bars indicate the frequency of TG-to-CA mutation, while red bars indicate perfect edits with no detectable mutations in the 36-nt sequence shown in panel c. Error bars represent s.d. from n = 6 replicates. f Indel activity in CD4+ human T cells following BhCas12b v4 RNP delivery. Each dot represents an individual electroporation (n = 2). Source data are provided as a Source Data file
