Fig. 1
miR-500a-5p is down-regulated in CRC and associated with malignant biological behaviour. a Representative heat map of the miRs that were most differentially expressed in both SW620 and LoVo cells compared with FHC cells. Each row represents an miR and each column represents a cell line. The experiment was performed in triplicate. Red represents up-regulation and green down-regulation, respectively. b Validation of miR-500a-5p expression levels in colon epithelial cell lines NCM460, FHC, SW480, DLD1, SW1116, SW620, HCT1116, LoVo and Caco2 cells by qPCR. One-way ANOVA and Dunnett’s T3 multiple comparison test. ****P < 0.001. c Real-time PCR analysis of miR-500a-5p expression in 81 pairs of human CRC tissues and their adjacent normal mucosal tissues. NT, normal mucosal tissues. CRC, colorectal cancer tissue. Error bars represent the mean ± SD from three independent experiments. d As analysed by qRT-PCR, miR-500a-5p expression in CRC tissues was significantly lower than that in the corresponding non-cancerous colon mucosa tissue. Student’s t test; **P < 0.05. e Representative ISH images of miR-500a-5p expression. f and g Effects of miR-500a-5p mimics or inhibitor on the proliferation of colon epithelial cells, as determined by colony formation assay. h and i DNA synthesis in LoVo cells was measured by the EdU incorporation assay at 48 h after the indicated transfection. Red fluorescence represents the EdU-positive cells; blue fluorescence from the Hoechst 33342 stain represents the total cells. These figures were representative of three to four independent experiments with identical results. Scale bars, 50 μm in (e) and 100 μm in (h) and (i)
