Fig. 2 | Nature Communications

Fig. 2

From: The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer

Fig. 2

miR-500a-5p directly targets HDAC2 in CRC. a The five-way Venn diagram indicates the numbers of genes that overlapped in four publicly available bioinformatics algorithms (miRanda, TargetScan, miRTP, RNA22-HSA) and the microarray-based miR-500a-5p signature. b The heat map was based on 60 candidate genes that were down-regulated in LoVo cells. Red color represents an expression level above mean, green color represents an expression lower than the mean. c and d HDAC2 protein and miR-500a-5p expression in ten freshly collected CRC biopsies using western blot and qRT-PCR analyses. e In human CRC tissues, HDAC2 was negatively correlated with miR-500a-5p expression. All results were expressed as the mean of three to four independent experiments ± SD. Linear regression analysis. r = –0.646, ****P < 0.001

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