Fig. 4
miR-500a-5p modulates CRC tumour growth by targeting HDAC2 in vivo. a Fluorescence images of subcutaneous tumours of mice injected with LoVo/m-NC, LoVo/miR-500a-5p, LoVo/Vector, LoVo/HDAC2, and LoVo/miR-500a-5p/HDAC2 cells. The growth of the resultant primary tumours was monitored. b Tumour size was measured starting from the 13th day after tumour cell inoculation in each group. Student’s t test; ***P < 0.01, m-NC vs miR-500a-5p and ***P < 0.01, miR-500a-5p vs miR-500a-5p/HDAC2. c–f Immunohistochemical (IHC) detection and quantification of Ki-67 and CD105 protein expression in subcutaneous tumours from mice injected with LoVo cells. Student’s t test; ***P < 0.01, m-NC vs miR-500a-5p and ***P < 0.01, miR-500a-5p vs miR-500a-5p/HDAC2 in Ki-67. ***P < 0.01, m-NC vs miR-500a-5p and ****P < 0.001, miR-500a-5p vs miR-500a-5p/HDAC2 in CD105. Scale bars, 200 μm in (c) and (e)
