Fig. 1 | Nature Communications

Fig. 1

From: Diverse motif ensembles specify non-redundant DNA binding activities of AP-1 family members in macrophages

Fig. 1

AP-1 proteins have overlapping and distinct transcriptional functions in macrophages. a Protein alignment of monomers (right) and mRNA expression of monomers in thioglycollate elicited macrophages before and after 1-h Kdo2 lipid A treatment (left). b Hierarchical clustering of genes that are differentially expressed in immortalized bone marrow-derived macrophages subjected to CRISPR-mediated knockdown of the indicated AP-1 monomer with respect to scramble control. Expression values are given as the fold change with respect to scramble; values are Z-score normalized across each row. Representative functional annotations for each gene cluster are calculated using Metascape and the enrichment of each term is quantified as the negative log transform of the p-value. c Expression of a subset of genes in AP-1 protein knockouts. n=2. End points of the error bars indicate the value from each replicate. * indicates False discovery rate (FDR) < 0.05

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