Fig. 7
The Jun-specific DNA binding program is preferentially altered in PPARγ knockout macrophages. a–c Changes in binding strength all binding sites in wild type macrophages in PPARγ-KO macrophages (left) and Venn diagrams summarizing the change in binding at binding sites that overlap with PPARγ (right) for Jun (a), ATF3 (b), and JunD (c). d Representative browser shots of Jun in WT and PPARγ-KO thioglycollate elicited macrophages (TGEMs) and PPARγ in WT TGEMs. e Western blot analysis of co-immunoprecipitation experiments between AP-1 monomers ATF3, Jun and JunD and PPARγ in TGEMs. f Western blot analysis of co-immunoprecipitation experiments between AP-1 monomers ATF3 and JunD and PPARγ in scramble immortalized bone marrow-derived macrophage (iBMDM) or CRISPR-mediated Jun knockout iBMDM
