Fig. 1
From: Regulation of substrate utilization and adiposity by Agrp neurons
Rapid shift in substrate utilization upon activation of agouti-related peptide (Agrp) neurons. a Food intake. b Respiratory exchange ratio (RER) (interaction: F45, 630 = 8.40, P < 0.0001; time: F45, 630 = 13.86, P < 0.0001; group: F1, 14 = 52.59, P < 0.0001). c Calculated fat utilization (interaction: F45, 630 = 6.44, P < 0.001; time: F45, 630 = 7.22, P < 0.0001; group: F1, 14 = 33.83, P < 0.0001). d Calculated carbohydrate utilization (interaction: F45, 630 = 2.77, P < 0.0001; time: F45, 630 = 18.48, P < 0.0001; group: F1, 14 = 16.73, P = 0.001). e Linear regression analysis between the size of the initial bout of food intake (first 30 min) and RER (at the 60 min measurement) in control and AgrpTrpv1 mice. f VO2 (interaction: F45, 630 = 1.07, P = 0.34; time: F45, 630 = 11.22, P < 0.0001; group: F1, 4 = 0.01, P = 0.89). g VCO2 (interaction: F45, 630 = 0.88, P = 0.68; time: F45, 630 = 15.07, P < 0.0001; group: F1, 14 = 1.58, P = 0.22). In f and g, inserts show the mean VO2 and VCO2. h Energy expenditure (interaction: F45, 630 = 0.96, P = 0.53; time: F45, 630 = 12.09, P < 0.0001; group: F1, 14 = 0.02, P = 0.87). i Ambulatory activity (interaction: F45, 630 = 0.80, P = 0.81; time: F45, 630 = 6.02, P < 0.0001; group: F1, 14 = 0.31, P = 0.58). Statistical analysis was performed using two-way analysis of variance (ANOVA) with time as a repeated measure followed by Holm–Sidak’s multiple comparisons test (MCT). Gray bars indicate time points in which MCTs were statistically significant (P < 0.05). From a–i control (black; n = 8) and AgrpTrpv1 mice (blue; n = 8). Dashed line indicates time of capsaicin injection. Symbols indicate mean ± SEM
