Fig. 8
From: A conserved dimer interface connects ERH and YTH family proteins to promote gene silencing
Mmi1W112A uncouples EMC-dependent and -independent Mmi1 functions. a 3′ RACE of ssm4 and leu1 (control) loci. Red triangles indicate termination sites as determined by sequencing of 3′ RACE products. The black arrow indicates the gene-specific forward primer used, which can be found in Supplementary Table 4. b RNA-seq expression profiles of prt-pho1 loci and nam1-byr2 in WT, mmi1W112A, erh1∆, and mmi1∆. Shown is normalized RPKM expression. c Northern blot analysis at the prt-pho1 locus in indicated strains using a probe targeting pho1 mRNA (top and bottom panels). d Northern blot analysis at the nam1-byr2 locus in indicated strains using a probe targeting nam1 ncRNA. Source data are provided as a Source Data file
